About this Event
Join us at Counter Culture Labs for a 2-day bootcamp where you will learn the fundamentals of CRISPR-Cas9 and make precision gene edits in E. coli.
CRISPR-Cas9 is a precision genetic engineering tool where guide RNA directs a Cas9 enzyme to a specific target to make a double-stranded break which can be repaired with template DNA. Not only will we genetically engineer microbes with CRISPR but also design our own edits and verify transformations with molecular biology techniques, including PCR and gel electrophoresis.
Program Details:
April 25 & 26 weekend from 10am - 4:30pm daily
Day 1
- History of CRISPR
- Plasmid DNA and inserting DNA into microbes
- Evolution of competence (techniques: heat shock, chemical, electroporation)
- Preparing electrocompetent cells and performing electroporation
- Selection vs screening and plating transformants
- Electronic lab notebook
Day 2
- Observing phenotypes
- Genotyping tools for verifying edits
- PCR to amplify transformant DNA
- Gel electrophoresis and sequencing
- Generating your own Cas9 sgRNA and template
- DNA design for inserting fluorescent proteins into microbes
- Advanced CRISPR applications and technologies
Required lab attire for safety: Long pants and closed-toed shoes.
Please bring a laptop so that you can participate in the DNA design lessons.
Unable to attend? Interested in other classes like plant genetic engineering? Join our mailing list to hear about upcoming Bay Area workshops!
About The Instructors
Jason Liu PhD is a translational biology scientist and manager specializing in leading teams for therapeutic, programmable large DNA integrations. He has 15+ years of expertise in accelerating novel CRISPR technologies to human cells, molecular biology, cell/strain engineering, and high throughput and NGS assay development.
Anthony Neil Tan is a UC Berkeley trained bioengineer whose projects center on enhancing high-value plants through genetic engineering. His past work ranges from designing a plant growth chamber that won a national NASA contest to developing new plant genetic engineering tools at the U.S. Department of Energy's Joint BioEnergy Institute.
No refunds can be issued.
Event Venue & Nearby Stays
549 48th St, 549 48th Street, Oakland, United States
USD 642.09
