About this Event
Join us at BioPunk Lab in SF for a 2-day bootcamp where you will learn the fundamentals of CRISPR-Cas9 and make precision genome edits in E. coli.
CRISPR-Cas9 is a precision genetic engineering tool where guide RNA directs a Cas9 enzyme to a specific genomic target to make a double-stranded break which will be repaired with template DNA. Not only will we genetically engineer microbes with CRISPR but also design our own edits and verify transformations with molecular biology techniques, including PCR and gel electrophoresis.
Program Details:
February 14-15 weekend from 10am - 4:30pm daily
6 spots total - RSVP Today
Day 1
- History of CRISPR
- Plasmid DNA and inserting DNA into microbes
- Evolution of competence (techniques: heat shock, chemical, electroporation)
- Preparing electrocompetent cells and performing electroporation
- Selection vs screening and plating transformants
- Electronic lab notebook
Day 2
- Observing phenotypes
- Genotyping tools for verifying edits
- PCR to amplify transformant DNA
- Using counterselection
- Gel electrophoresis and sequencing
- Generating your own Cas9 sgRNA and template
- DNA design for inserting fluorescent proteins into microbes
Required lab attire for safety: Long pants and closed-toed shoes. Unable to attend? Interested in other classes like plant genetic engineering? Join our mailing list to hear about upcoming Bay Area workshops!
Parking garage nearby (in case street parking is unavailable):
440 Stevenson Street, San Francisco, CA 94103
About The Instructors
Jason Liu PhD is a translational biology scientist and manager specializing in leading teams for therapeutic, programmable large DNA integrations. He has 15+ years of expertise in accelerating novel CRISPR technologies to human cells, molecular biology, cell/strain engineering, and high throughput and NGS assay development.
Anthony Neil Tan is a UC Berkeley trained bioengineer whose projects center on enhancing high-value plants through genetic engineering. His past work ranges from designing a plant growth chamber that won a national NASA contest to developing new plant genetic engineering tools at the U.S. Department of Energy's Joint BioEnergy Institute.
No refunds can be issued.
Event Venue & Nearby Stays
BioPunk Lab (8th Floor of Frontier Tower), 995 Market Street, San Francisco, United States
USD 642.09
