VaxTech Fridays (Nanotemper - Eiyong Ahn)

Title: New possibilities on molecular interaction and in-depth characterization of protein studies
About this Event
Event Photos

Speaker Bio:

Dr. Eiyong Ahn

TSS specialist Korea

NanoTemper Technologies : Seoul/Korea

- NanoTemper Technologies: 2021 – now:

o Main responsibilities: Specialist and Support for all instrument lines of NanoTemper. Close collaboration with our Korean channel partners (HnS Bio) and management of our customer support- and demo-laboratory in South Korea.

- PhD: 2017 – 2021 in Molecular Biology:

o Where: Germany / FAU

o Topics: HIV-1 and HIV-2 differ greatly in the activation and secretion of ADAM protease through plasma extracellular vesicles (pEV)

- Research assistant: 2013 - 2016

o Where: USA / University of Wyoming

o Topics: Conducted experiments to achieve microbiology research objectives. Mostly related with light enduced protein functions. Purified genomic DNA. Directed several molecular cloning projects.

- Masters degree: 2009 - 2012 in Molecular Biology:

o Where: Korea / Yonsei University

o Topics: RASSF1A-mediated Regulation of AREG via the Hippo Pathway in Hepatocellular Carcinoma

Abstract:

NanoTemper Technologies has developed a novel approach to the characterization of molecular interactions: Spectral Shift. This new technology is the driving engine for our latest product releases Dianthus and Monolith X. Both instruments feature a combination of the new isothermal spectral shift detection with the established MST/TRIC technology. The instruments enable characterization of in solution interactions for a wide range of biomolecules, even for challenging samples such as PROTACs, membrane proteins, intrinsically disordered proteins, and cell lysates. The instruments enable mass-independent, in-solution measurements with lowest sample consumption and even in the presence of aggregation. Dianthus and Monolith X can not only be used to validate your results from other biophysical methods but also to characterize your most challenging interactions. Don’t miss out on the opportunity to learn about this game-changing new biophysical assay technique.

NanoTemper Technologies has developed the Prometheus which the scientific community has chosen because it consistently delivers high-resolution data that reveals details in proteins and formulations that are missed by other technologies. The latest model (Prometheus Panta) is equipped with the current gold standard for protein stability characterization (label free nanoDSF + backreflection), and a novel type of DLS/SLS enabling new experimental possibilities.

Besides standard applications, the new type of DLS/SLS now allows dynamic light scattering (DLS) experiments in tandem with static light scattering (SLS), label free nano-differential scanning fluorimetry (nanoDSF) and backreflection, along an entire thermal ramp, to gain information on conformation, thermal stability, turbidity, radius, size distribution and more, on your protein of interest or formulation.

In this presentation the measurement principles of Prometheus Panta and several case studies will be shown that demonstrate how i.e. Merck Healthcare KGaA, Novartis, Boehringer Ingelheim and others use the standard and new possibilities of Panta for in-depth characterization and QC of biologics, gene therapy vectors and more.